Screening of genes associated with methylglyoxal resistance in escherichia coli = Methylglyoxal 내성과 연관된 대장균 유전자의 탐색

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Methylglyoxal (MG) acts as a toxic material in various species, known as a reactive α-oxoaldehyde having potential risk in disease. But it can be degraded by the in vivo biochemical pathway. Genes related to methylglyoxal were screened in Escherichia coli MG1655 strain. Among them, five unknown genes, yahA, yjhT, ydbD, yqiI, and yjjQ were chosen as candidates. Deletions of these genes showed lethality to methylglyoxal and dihydroxyacetone (DHA) in minimal media. When crude extracts of the deleted strains were assayed to detect methylglyoxal conversion activity by NMR, MG1655ΔgloΔyqiI exhibited reduced acetol and lactate productions. MG1655ΔgloΔydbD also showed lower level of lactate production than that of MG1655Δglo, suggesting that ydbD plays a role in the pathway to lactate production.
Advisors
Park, Chan-Kyuresearcher박찬규researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2006
Identifier
255232/325007  / 020043359
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2006.2, [ 4, 35 p. ]

Keywords

Methylglyoxal; hydroxyacetone; Methylglyoxal 내성; yqiI; lactate

URI
http://hdl.handle.net/10203/28069
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=255232&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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