Isolation of cDNAs encoding the pyruvate dehydrogenase and citrate synthase from tomato = 토마토의 Pyruvate dehydrogenase와 citrate synthase의 cDNA 클로닝 및 분석

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Two cDNAs encoding pyruvate dehydrogenas(PD) and citrate synthase(CS) related to citrate were isolated and characterized from tomato plants. Two clones PD, CS were 1416 bp and 1176 bp, respectively and had a open reading frames(ORF), deriving 52kDa and 43kDap olypeptide. The identities between the deduced amino acid sequences of tomato CS and potato CS were 98%. For PD of tomato and potato same identities (98%) were shown. Genomic DNA blot analysis suggests that MD,PD and CS may be single copy genes. The high est expression of PD, CS and MD (malate dehydrogenase) occurred in roots and MD, PD and CS were expressed in fruit at all developmental stages, and their expression levels were relatively similar. The present study suggests that the expression of TCA cycle was not uniform in the different tissues of the tomato. Although mitochondrial citrate synthase and pyruvate dehydrogenase are usually described as a housekeeping enzyme, its expression is developmentally regulated, indicating that in certain tissues or under certain environmental conditions a higher amount of the enzyme is required.
Advisors
Chung, Won-Ilresearcher정원일researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2000
Identifier
158708/325007 / 000983475
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 2000.2, [ iv, 40 p. ]

Keywords

Malate dehydrogenase; Citrate synthase; Pyruvate dehydrogenase; Tomato; Citrate; 시트르산; 말레이트 디하이드로제나제; 시트르산 신세이즈; 피루브산 디하이드로제나제; 토마토

URI
http://hdl.handle.net/10203/27908
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=158708&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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