Production of single cell protein from methanol : optimization of medium and maximization of biomass productivity = 메탄올을 이용한 미생물단백질의 생산에 관한 연구 optimization of medium and maximization of biomass productivity

A $\mbox{\underline{Methanomonas}}$ Sp. that can utilize methanol as a sole carbon source has been isolated from the local soil samples in Korea. By using this organism, a continuous fermentation process was developed for the production of single cell protein. For this process, the medium and the process operating conditions have been optimized for the maximum biomass productivity by using continuous culture technique. This organism is gram-negative rod, 0.5 to 1.0 ㎛ wide and 1.5 to 2.0 ㎛ long, and occurs in pair. The maximum specific growth rate of this organism was 0.4 $hr^{-1}$ at PH 7.3 and 30 ℃. In a carbon-and nitrogen-limited chemostat, yield coefficient for carbon source and nitrogen source were 0.4 gram-cell per gram-methanol and 6.79 gram-cell per gram-ammonia respectively at a dilution rate of 0.2 $hr^{-1}$. The maximum biomass productivity obtained was 0.62 gram-cell/ liter-hour at a dilution rate of 0.2 $hr^{-1}$. Protein content of the single cell protein produced by this process was 71.5%, and amino acid profile in terms of the essential amino acid was as good as or better than FAO standard for food protein.
Advisors
Ryu, Dewey D.Y.유두영
Publisher
한국과학기술원
Issue Date
1976
Identifier
61958/325007 / 000741018
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1976.2, [ vii, 73 p. ]

URI
http://hdl.handle.net/10203/27762
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=61958&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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