Cytotoxicity of V79 cell and change of DNA structure by 6-sulfooxymethyl benzo(a)pyrene = V79세포에서 6-sulfooxymethyl benzo(a)pyrene 의 독성 시험과 이 물질이 DNA 구조에 미치는 영향

Effect of 6-sulfooxymethyl benzo(a)pyren (SMBP), an ester form of benzo (a)pyrene metabolite, on cytotoxicity and on change of DNA structure were investigated. Relative survival rate was linearly decreased within the range of 1.0$\mu$M SMBP. Glutathione S-transferase (GST) and vitamin C reduced the cytotoxicity of SMBP in dose dependent manner and it appears that GST detoxify damage by SMBP more effectively than Vitamin C. Supercoiled DNA was changed to relaxed and linear DNA by treatment of SMBP. The result suggest that SMBP nicks the plasmid and supercoil DNA can be cleaved to small DNA fragments. 6-Sulfooxymethyl benzo(a) pyren keeps B-DNA conformation because the transition of B-Z could be related to regulation of gene. When calf thymust DNA was incubated with SMBP, the major DNA adduct was identified as the deoxyguanosine. This DNA adducts may cause cytotoxicity and further mutagenicity.
Advisors
Chung, An-Sikresearcher정안식researcher
Publisher
한국과학기술원
Issue Date
1992
Identifier
60376/325007 / 000901528
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ vi, 47 p. ]

URI
http://hdl.handle.net/10203/27760
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60376&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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