Chemical modification of red blood cells and hemoglobins with methoxy-polyethylene glycol = 메톡시-폴리에틸렌 글리콜에 의한 적혈구와 헤모글로빈의 화학적 수정

Because mPEG modified proteins show both reduced antigenic and immunogenic properties and extended blood circulating life times, attachment of polyethylene glycol to proteins has found wide applications for biomedical and biotechnological purposes. In this experiments. Sheep Red blood Cells and Sheep Hemoglobins were modified with mPEG to develop the potential blood substitute. Osmotic fragility of SRBC-mPEG conjugates was determined by degree of hemolysis against salt concentrations, and antigenicity of SRBC-mPEG conjugates was determined by Blood grouping and Cross-matching test. mPEG modified SRBCs show no aggregation by blood grouping and cross-matching test, and are resistant to osmotic hemolysis. mPEG modified SHb show altered electrophoretic mobility, and modification degrees of the conjugates were calculated from TNBS free amino group titration results. Oxygen carrying capacity of SHb-mPEG conjugates was determined by tonometrically. 6.3\% modified conjugate show increased oxygen affinty and increased saturation end point. Antigenicity of SHb-mPEG conjugates was determined by double immunodiffusio test. mPEG-SHb conjugate show no antigenicity at 34\% of the free amino groups modified. But, only slightly modified mPEG-SHb conjugates (6.3\%) show oxygen transport activity similar to unmodified hemoglobin.
Advisors
Byun, Si-Myungresearcher변시명researcher
Publisher
한국과학기술원
Issue Date
1992
Identifier
60373/325007 / 000901488
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ vii, 42 p. ]

URI
http://hdl.handle.net/10203/27757
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60373&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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