Mutagenesis of T7 RNA polymerase = T7 RNA 중합 효소의 돌연변이 유도

For structural analysis of the phage T7 RNA polymerase, the phagemid pKU 01 carrying plasmids the T7 RNA polymerase gene under the control of lac promoter was constructed from pAR1219 and pUC119. Using recently developed, efficient random mutagenesis method of Xiube Liao, the C-terminal region of the T7 RNA polymerase was mutagenized. For a more efficient mutagenesis, a higher concentration of misincorporating nucleotide and longer misincorporation reaction time. As results a library of E. coli colonies containing mutations in the T7 RNA polymerase gene was constructed. Occurrance of mutations was suggested by the fact that colonies were formed when the mutated plasmids were inserted in E. coli BL21(DCAT4), which would not grow with wild-type T7 RNA polymerase gene.
Advisors
Kang, Chang-Wonresearcher강창원researcher
Publisher
한국과학기술원
Issue Date
1992
Identifier
60369/325007 / 000901441
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ iv, 39 p. ]

URI
http://hdl.handle.net/10203/27753
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60369&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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