Induction of cytochrome P-450s by 2-acetylaminofluorene in primary cultures of adult rat hepatocytes = 일차 배양 간세포에서 2-Acetylaminofluorene에 의한 cytochrome P-450의 유도

The induction of cytochrome P-450 by 2-acetylaminofluorene, a potent hepatocarcinogen, was studied in primary cultures of adult rat hepatocytes. Addition of 2-acetylaminofluorene to the hepatocyte cultures induced the P-450IA 1-specific ethoxyresorufin O-deethylase activity in dose- and time-dependent manner. The P-450IIB-specific pentoxyresorufin O-dealkylase activity was also induced, though to an even lesser degree, at high concentration of 2-acetylaminofluorene. To elucidate the mechanism of the induction of ethoxyresorufin O-deethylase activity by 2-acetylaminofluorene, we performed Northern blot analysis using a specific oligonucleotide probe recognizing P-450IA 1/2 mRNA and found that the induction is not mediated by the accumulation of the specific mRNA. The result of experiment using cycloheximide, an inhibitor of protein biosynthesis, in which 0.5$\mu$M cycloheximide completely blocked the induction of P-450IA1-specific ethoxyresorufin O-deethylase activity indicated that protein biosynthesis is required for the induction in response to 2-acetylaminofluorene and this was supported by the enzyme-linked immunosorbent assay showing actual increase in P-450IA1 protein.
Advisors
Yang, Kyu-Hwanresearcher양규환researcher
Publisher
한국과학기술원
Issue Date
1992
Identifier
60368/325007 / 000901433
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ [iii], 41 p. ]

URI
http://hdl.handle.net/10203/27752
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60368&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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