Vesicle binding and structural properties of luteinizing hormoe releasing hormone (LHRH) = LHRH 의 막 결합 성질과 구조적 성질에 관한 연구

The interaction between phosphatidylserine multilamellar vesicle (PSMLV) and synthetic luteinizing hormone releasing hormone (LHRH) was investigated by intrinsic fluorescence and centrifugal separation of PS MLV-LHRH complexes. PS MLV induced a blue-shift in the wavelength of maximum emission of fluorescence of LHRH by about 7 nm. The influence of PS MLV on the quenching of the tryptophan in LHRH by sodium iodide (NaI) was studied. PS MLV was found to protect LHRH from the iodide quenching. The conformation of LHRH was investigated by circular dichroic measurements. The Greenfield-Fasman and the Chang-Wu-Yang methods were used for the analysis of CD spectra. It was found that LHRH has appreciable $\beta$-structure even in aqueous solution. The $\beta$-strcture content increased significantly upon binding to amphihpilic surface.
Advisors
Kim, Hyoung-Manresearcher김형만researcher
Publisher
한국과학기술원
Issue Date
1992
Identifier
60354/325007 / 000901191
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ v, 49 p. ]

Keywords

황체 형성 호르몬 분비 호르몬

URI
http://hdl.handle.net/10203/27738
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60354&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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