Molecular cloning and nucleotide sequence analysis of a Hsp70 cDNA in a mouse fibroblast = 생쥐 섬유아세포에서 70 kDa 고온충격 단백질 cDNA 유전자의 클로닝과 DNA 염기서열 분석

Heat shock protein 70 (hsp 70), is the major protein expressed when cells are heat-shocked. We tried to isolate hsp 70 cDNA from mouse fibroblast ID13. ID13 cDNA library was screened with human hsp70 gene as a probe, and a positive clone was obtained. The positive clone was subcloned into pUC19 and the precise restriction map was obtained. The cDNA gene was sequenced by Sanger dideoxy termination method. A single long open reading frame that could code for a protein of 643 amino acids was found. The sequence of cloned mouse hsp70 shows great homology (60-97\%) with other mouse hsp 70 genes and somewhat less homology (50\%) with E. coli hsp 70 gene (dnaK). The great homology (97\%) with already cloned mouse heat shock cognate 70 kDa protein (hsc 70) make us to think that the cloned gene is constitutively expressed at normal temperature.
Advisors
Choe, Joon-Horesearcher최준호researcher
Publisher
한국과학기술원
Issue Date
1992
Identifier
60347/325007 / 000901131
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1992.2, [ vi, 57 p. ]

URI
http://hdl.handle.net/10203/27731
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60347&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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