Effect of deglycosylation on 7S protein soybean seed = 탈당화가 대두종의 7S 단백질의 특성에 미치는 영향에 관한 연구

7S-globulin was purified by using ion-exchange chromatography (DEAE-Sephadex A-50) and affinity chromatography (Con-A Sepharose 4B) from defatted soy flour to study the function of its glycosidic residues. The glycosidic residue was removed by trifluoromethane sulfonic acid treatment without protein denaturation. The properties of deglycosylated derivative were examined and compared with the native one. The heat stability of deglycosylated derivative was decreased and the solbility behavior as a function of pH was changed. The effect of NaCl concentration on solubility was varied. Also the viscosity and digestibility against $\alpha$ -Chymotrypsin was changed.
Advisors
Rhee, Joon-Shickresearcher이준식researcher
Publisher
한국과학기술원
Issue Date
1991
Identifier
59999/325007 / 000891072
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1991.8, [ v, 32 p. ]

Keywords

단백질 분리.

URI
http://hdl.handle.net/10203/27727
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=59999&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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