Inhibition of MK5-mediated cell migration by $14-3-3\epsilon$ = MK5에 의한 세포 이동에 미치는 14-3-3ε의 영향

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The signal pathway by which 14-3-3ε inhibits cell migration induced by MAPK-activated protein kinase 5 (MK5) was investigated in cultured HeLa cells. Both $in vivo$ and $in vitro$ analyses have revealed that 14-3-3ε interacts with MK5. Other MKs including MK2 and MK3 appeared to fail to interact with 14-3-3ε. 14-3-3ε bound to MK5 inhibits the phosphorylation of HSP27, a known substrate of MK5. Disturbance of actin cytoskeleton organization by 14-3-3ε was shown in transfected cells transiently expressing 14-3-3ε as well as established cells stably expressing 14-3-3ε. Moreover, overexpression of 14-3-3ε resulted in the inhibition of cell migration induced by MK5 overexpression or TNFα treatment. Results in this study suggest that 14-3-3ε bound to MK5 inhibits cell migration by inhibiting the phosphorylation of HSP27 whose phosphorylation regulates F-actin polymerization, actin cytoskeleton organization and subsequent actinfilament dynamics.
Advisors
Joe, Cheol-O.researcher조철오researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2008
Identifier
295336/325007  / 020035890
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2008.2, [ viii, 94 p. ]

Keywords

MK5; 14-3-3ε; HSP27; cell migration; F-actin polymerization; MK5; 14-3-3ε; HSP27; 세포이동; F-액틴 중합반응; MK5; 14-3-3ε; HSP27; cell migration; F-actin polymerization; MK5; 14-3-3ε; HSP27; 세포이동; F-액틴 중합반응

URI
http://hdl.handle.net/10203/27641
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=295336&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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