Expression and functional analysis of human UREB1 in human colorectal carcinoma = 인체 대장암 조직 및 세포에서의 인간 UREB1 유전자의 발현 및 기능에 관한 연구

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The protein human upstream regulatory element binding protein 1(hUREB1) is a HECT domain protein whose function is not defined yet. Here, we investigate the function of hUREB1 as a ubiquitin-protein ligase in human colorectal cells. Ectopic expression of the HECT domain of hUREB1 reduces the protein level and transcriptional activity of the p53 tumor suppressor, which is inhibited by the deletion in the HECT domain or point mutations in the essential residues of the HECT domain, implying that the HECT domain of hUREB1 suppresses the transcriptional activity of p53 through a ubiquitin-dependent degradation pathway. Based on the results of Northern blot analysis, RT-PCR and immunohistochemical analyses, the over-expression of hUREB1 is associated with colorectal carcinoma. These findings indicate that hUREB1 can function as a negative regulator of p53 during the colorectal carcinoma progression through the ubiquitination pathway mediated by the HECT domain.
Advisors
Chung, An-Sik정안식
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
245030/325007  / 000995821
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2005.2, [ xi, 105 p. ]

Keywords

UREB1; human; colorectal; antibody; CRC tissues; 인간 UREB1 대장암 p53 단백질; carcinoma

URI
http://hdl.handle.net/10203/27592
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=245030&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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