Biochemical role of pig heart mitochondrial creatine kinase = 돼지 심장 미토콘드리아에 존재하는 크레아틴 키아제의 생화학적 역할

In order to elucidatate the biochemical role of heart mitochondrial creatine kinase in the energy metabolism of heart muscle cell, we studied the mechanism on the coupling between the production of phosphocreatine by respiring heart mitochondria and oxidative phosphorylation. We also studied the effect of the concentration ratio of phosphocreatine to creatine on the rate of coupled respiration as well as that of uncoupled respiration in order to examine the possible regulatory role of the creatine kinase in the energy metabolism. The followings are the main results. 1. Effect of the concentration ratio of phosphocreatine to creatine on the rate of respiration: In the absence of added creatine and phosphocreatine, pig heart mitochondria respiring in the presence of glutamate and malate exhibited an approximately 5-fold increase in the initial State 4 respiratory rate upon the addition of ADP, followed by return to the state of the Post-ADP State 4 rate. When the concentration of creatine was increased, the rate of the State 3 as well as the post-ADP State 4 increased further. The increased rates of the State 4 and 4 respiration due to the addition of creatine were diminished in the presence of phosphocreatin. The half maximal stimulation of post-ADP State 4 respiration was obtained with 8 mM creatine, which is comparable to the value of apparent Km of purified pig heart mitochondrial creatine kinase for creatine(8.5 mM). The State 3 respiratory rate decreased by increasing the concentration ratio of phosphocreatine to creatine with the total concentration of creatine plus phosphocreatine fixed at 20 mM. The maximum state 3 respiratory rate in the presence of excess ADP was further stimulated about 2-fold by the addition of 20 mM creatine. Also, the response of the uncouped respiratory rate in the presence of FCCP to the creatine and phosphocreatine was similar to that of the State 3 rate. These results indicate that the concentration ratio of phosphocreatine to ...
Advisors
김형만researcher
Publisher
한국과학기술원
Issue Date
1987
Identifier
60999/325007 / 000785047
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생물공학과, 1987.2, [ xii, 98 p. ]

URI
http://hdl.handle.net/10203/27255
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60999&flag=t
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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