DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Je-Kyun | - |
dc.contributor.advisor | 박제균 | - |
dc.contributor.author | Kim, Min-Seok S. | - |
dc.contributor.author | 김민석 | - |
dc.date.accessioned | 2011-12-12T07:28:27Z | - |
dc.date.available | 2011-12-12T07:28:27Z | - |
dc.date.issued | 2006 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=255490&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27120 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2006.2, [ ix, 60 p. ] | - |
dc.description.abstract | This paper describes a novel microfluidic cell culture platform for 3-dimensional cell patterning and in situ cell-based assays. The microfluidic device is fabricated by photolithography and poly(dimethylsiloxane) (PDMS) replica molding. The mixture of HepG2 cells and a sol-gel transition peptide hydrogel is hydrodynamically focused and patterned in the middle of the main channel of a microfluidic device. The cells are 3-dimensionally encapsulated and cultured on a microscope plate for over 2 days at the same time. A toxicant injected into one side inlet of a microfluidic device shows a linear concentration gradient profile at the entire area in the stripe-shaped micro-scaffold. The higher toxicant concentration gradient forms, the wider dead zone of cells in the peptide scaffold represents. This microfluidic platform facilitates in vivo-like 3-dimensional microenvironment, and provides credible cell-based screening and assays including cytotoxicity test, cell viability monitoring, continuous dose-response assay, drug-drug interaction and metabolism analysis. The device is also contributed to a novel biomimetic blood vessel platform for micro-angiological study by exchanging the mixture and media flows. Various geometries of vessel walls and blood flow in a biomimetic blood vessel model can be controlled by the media, distilled water and media flows in a microfluidic device. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 3-dimensional cell culture | - |
dc.subject | Microfluidics | - |
dc.subject | cell-based assays | - |
dc.subject | 세포기반분석 | - |
dc.subject | 삼차원 세포배양 | - |
dc.subject | 미세유체 | - |
dc.title | Development of a microfluidic 3-dimensional cell culture platform for cell-based assays | - |
dc.title.alternative | 미세유체조절에 의한 삼차원 세포배양 플랫폼의 개발과 세포기반분석 응용 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 255490/325007 | - |
dc.description.department | 한국과학기술원 : 바이오시스템학과, | - |
dc.identifier.uid | 020043073 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.contributor.localauthor | 박제균 | - |
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