Development of a microfluidic 3-dimensional cell culture platform for cell-based assays = 미세유체조절에 의한 삼차원 세포배양 플랫폼의 개발과 세포기반분석 응용

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 215
  • Download : 0
This paper describes a novel microfluidic cell culture platform for 3-dimensional cell patterning and in situ cell-based assays. The microfluidic device is fabricated by photolithography and poly(dimethylsiloxane) (PDMS) replica molding. The mixture of HepG2 cells and a sol-gel transition peptide hydrogel is hydrodynamically focused and patterned in the middle of the main channel of a microfluidic device. The cells are 3-dimensionally encapsulated and cultured on a microscope plate for over 2 days at the same time. A toxicant injected into one side inlet of a microfluidic device shows a linear concentration gradient profile at the entire area in the stripe-shaped micro-scaffold. The higher toxicant concentration gradient forms, the wider dead zone of cells in the peptide scaffold represents. This microfluidic platform facilitates in vivo-like 3-dimensional microenvironment, and provides credible cell-based screening and assays including cytotoxicity test, cell viability monitoring, continuous dose-response assay, drug-drug interaction and metabolism analysis. The device is also contributed to a novel biomimetic blood vessel platform for micro-angiological study by exchanging the mixture and media flows. Various geometries of vessel walls and blood flow in a biomimetic blood vessel model can be controlled by the media, distilled water and media flows in a microfluidic device.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 : 바이오시스템학과,
Publisher
한국과학기술원
Issue Date
2006
Identifier
255490/325007  / 020043073
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2006.2, [ ix, 60 p. ]

Keywords

3-dimensional cell culture; Microfluidics; cell-based assays; 세포기반분석; 삼차원 세포배양; 미세유체

URI
http://hdl.handle.net/10203/27120
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=255490&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0