Light-harvesting alginate hydrogel for sustainable photobiocatalysis = 광수확 알긴산 하이드로젤을 이용한 지속 가능한 생체촉매 기반 광합성 연구

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We report visible light-driven, asymmetric hydrogenation of C=C bonds using an ene-reductase from Thermus scotoductus SA-01 (TsOYE) and a light-harvesting dye (rose bengal, RB) co-immobilized in alginate hydrogel. Highly efficient encapsulation of RB in alginate hydrogel was achieved using the intrinsic affinity between TsOYE and RB, which allowed for the construction of robust RB-TsOYE-loaded alginate capsules. In the absence of NADH, the photobiocatalytic system facilitated asymmetric reduction of 2-methylcyclohexenone to an enantiopure (R)-2-methylcyclohexanone (ee>99%, max. conversion: 70.4%, turnover frequency: 1.54 $min^{-1}$, turnover number: 300.2) under illumination. A series of stability tests revealed a significant enhancement of TsOYE's robustness in alginate hydrogel against heat and chemical denaturants. This study provides insight into a greener and sustainable approach of cofactor-free OYE catalysis for producing value-added chemicals using light energy.
Advisors
Park, Chan Beumresearcher박찬범researcher
Description
한국과학기술원 :신소재공학과,
Publisher
한국과학기술원
Issue Date
2019
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 신소재공학과, 2019.2,[iv, 38 p. :]

Keywords

photobiocatalysis▼aene-reductase▼aasymmetric hydrogenation▼aNADH-free bioprocess▼aalginate hydrogel; 구황색 효소▼a탄소 이중결합 비대칭 환원▼a광생체촉매반응▼a니코틴아마이드 보조인자 부재 생체촉매반응▼a알긴산 하이드로젤

URI
http://hdl.handle.net/10203/266452
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=843308&flag=dissertation
Appears in Collection
MS-Theses_Master(석사논문)
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