Engineering of Escherichia coli for high-level production of styrene from glucose = 포도당으로부터 스티렌 고생산을 위한 대장균의 개량

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In the past few decades, global styrene production has been increasing due to styrene’s diverse use as the main building-block for various industrial styrene-derived polymers. Conventionally, the industrial supply for styrene has solely relied on the chemical synthesis from ethylbenzene. As an alternative strategy, biosynthesis of styrene has been demonstrated in various models including E. coli. However, previous studies have mostly focused on the pathway construction itself, or optimizing the culture conditions in order to deal with the end-product’s toxicity, leading to a relatively poor productivity. This work aimed to increase the production of styrene towards an applicable level in industry. In order to provide maximum flux towards the synthesis pathway, high-trans-cinnamic acid producer, obtained based on metabolic engineering, was applied. Also, production system was optimized by regulating enzyme expression levels an optimizing the culture media. From this, 1.9 g/L of styrene was produced from flask cultures. Finally, fed-batch cultures performed and optimized in order to further enhance the production titer. As a result, a maximum titer of 5.26 g/L was achieved by fed-batch cultures.
Advisors
Jeong, Ki Junresearcher정기준researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2019
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2019.2,[iv, 38 p. :]

Keywords

Styrene▼atrans-cinnamic acid▼aL-Phenylalanine▼aescherichia coli▼abiosynthesis▼afed-batch cultivation; 스티렌▼a계피산▼a페닐알라닌▼a대장균▼a생합성▼a유가식 배양

URI
http://hdl.handle.net/10203/266388
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=843729&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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