Enhancement of fatty acid methyl ester production in Chlorella sp. HS2 by overexpression of endogenous bZIP transcription factor = 전사인자 과발현을 통한 클로렐라의 지방산 메틸 에스터 생산성 향상 연구

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Biofuel production from microalgae is considered as a promising strategy for alternative and eco-friendly energy production, but is still challenged by problems including economic deficit. These problems can be solved by genetic modifications resulting in increased lipid productivity. Transcription factors (TFs) are emerging as candidates of genetic engineering of microalgae since they are involved in various metabolic pathways in organism. Among transcription factors, we investigated basic Leucin Zipper (bZIP) in Chlorella sp. HS2 since bZIP family is known as a key regulator of lipid biosynthesis in plants and other microalgae. In this study, we overexpressed HSbZIP1 TF and transformants showed enhanced biomass and lipid biosynthesis. In addition, we confirmed that expression levels of genes regulated by HSbZIP1 TF were up-regulated consistently during cultivation. Taken together, these results suggest that HSbZIP1 TF also plays an important role in lipid biosynthesis of Chlorella sp. HS2.
Advisors
Chang, Yong Keunresearcher장용근researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2019
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2019.2,[iv, 46 p. :]

Keywords

Microalgae▼aTranscription factor engineering (TEE)▼aBasic Leucin Zipper (bZIP)▼aFatty acid methyl ester (FAME); 클로렐라▼a비집 전사인자▼a지방산 메틸 에스터▼a바이오매스 생산성

URI
http://hdl.handle.net/10203/266383
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=843244&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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