Automatic metabolic reconstruction of disease-specific human microbiota – colorectal cancer as an example = 자동화 된 질병 관련 인체 장내미생물의 대사 모델 구축 및 대장암에 대한 적용 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 11
  • Download : 0
Human gut microbiota is ecological community of commensal microorganisms, which mediates human metabolisms. Recent studies have reported pathologic effects by changes in gut microbiota composition. However, microenvironment of intestinal lumen derived by gut microbiota metabolism have not well elucidated, which is required to develop personal therapeutic strategy for gut microbiota-related diseases. In this study, a platform was developed to collect personal taxon abundance dataset, automatically reconstruct genome-scale metabolic models (GEMs) of personal gut microbiota, and predict metabolic flux distribution of gut microbiota GEMs. For demonstration, 408 taxon abundance data of a colon cancer study were analyzed, and personal GEMs of gut microbiota were reconstructed. As a result, we found 9 taxa enriched in gut microbiota of colon cancer, including Staphylococcus aureus and Peptostreptococcus anaerobius, and altered metabolism by these species in gut microbiota GEMs of colon cancer. Also, using human context-specific GEMs, metabolic flux of colon cancer was analyzed, and we found possible metabolites related to colon cancer development. We ensure that this platform can be used to investigate metabolism of personal microbiota, eventually to find therapeutic strategy such as pre/probiotics.
Advisors
Lee, Sang Yupresearcher이상엽researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2019
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2019.2,[iv, 47 p. :]

Keywords

gut microbiota▼ametabolic modeling▼ametabolic flux prediction▼acolorectal cancer; 장내 미생물▼a대사 모델링▼a대사 흐름 예측▼a대장암

URI
http://hdl.handle.net/10203/266340
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=843197&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0