Quantitative analysis of RNA processing for clinical application = RNA 프로세싱의 임상용 정량분석법 개발

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 12
  • Download : 0
ARS-interacting multifunctional protein 2 (AIMP2) is a non-enzymatic component required for the assembly of the multi-tRNA synthetase complex. Yet, it also serves as a multifunctional tumor suppressor. While exon 2 skipping variant of AIMP2 (AIMP2-DX2) is associated with carcinogenesis, its potential as a cancer biomarker remains uninvestigated. Here, we combine RNA in situ hybridization (RNA-ISH), quantitative image-analysis, and RNA-sequencing to develop clinically applicable detection tools that can quantitate the expression ratio of AIMP2-DX2 to AIMP2. We apply our technique to analyze AIMP2-DX2 expression levels in both cell lines and patients’ samples. Our image quantification results show excellent concordance with pathologist’s reading and tissue-matched RNA-sequencing. We further employ our tool and find that Alu-binding proteins, especially ADAR and PKR, can regulate AIMP2-DX2 expression. Collectively, we provide the development and application of RNA-ISH image-analysis tools for quantitative analysis of AIMP2-DX2 and subclassification cancer patients based on AIMP2-DX2 expression.
Advisors
Kim, Yoosikresearcher김유식researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2019
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2019.2,[iii, 36 p. :]

Keywords

AIMP2▼aalternative splicing▼aRNA-ISH▼adiagnosis▼aalu-binding protein; AIMP2▼a선택적 스플라이싱▼aRNA-현장부합법▼a진단▼aAlu-결합 단백질

URI
http://hdl.handle.net/10203/266304
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=843198&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0