Characterization and control of apolipoprotein E fibril formation = 아포지단백질 E 섬유 형성의 특성 및 조절 연구

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Apolipoprotein E (apoE) is one of the strongest risk factors of Alzheimer’s disease (AD). ApoE is known to participate in the fibril formation of amyloid β in isoform-dependent manner. Due to its amphipathic nature, apoE can possess various structural arrangements including lipidated ‘open form’ and lipid-free ‘closed form’. In this thesis, the nanoscale structures of apoE were identified using biophysical techniques such as transmission electron microscopy (TEM), synchrotron small angle X-ray scattering (SAXS), and atomic force microscopy (AFM). At high concentration (> 0.1 mg/mL), apoE existed as tetramer, which appeared as ‘short rod’ shape. Interestingly, when apoE is diluted to physiological concentration (0.01-0.05 mg/mL), it formed elongated fibril structure. This fibrillation also occurred at chemically denaturing condition and high ionic strength, suggesting that ‘open form’ of apoE can be a prerequisite of fibrillation. This finding provides new insights on apoE self-association mechanism and its role in AD pathology.
Advisors
Choi, Myung Chulresearcher최명철researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2018.2,[v, 95 p. :]

Keywords

Apolipoprotein E▼aprotein fibrillation▼aalzheimer's disease▼ananostructure; 아포지단백질 E▼a단백질 섬유화▼a알츠하이머병▼a나노구조

URI
http://hdl.handle.net/10203/266195
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=867949&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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