Cultivation of Aurantiochytrium sp. KRS101 using jerusalem artichoke hydrolysate for DHA production = 돼지감자 당화액을 이용한 오란티오키트리움 KRS101 배양 및 DHA 생산에 관한 연구

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In the present study, Jerusalem artichoke (JA) was used as the sole carbon and nitrogen source for Aurantiochytrium sp. KRS101, a docosahexaenoic acid (DHA)-producing microalga. JA is known as non-food crop containing 70% of carbohydrate in a form of inulin which is easily degraded into fructose. A response surface methodology (RSM) analysis combined with experimental validation revealed that the maximum DHA productivity was obtained in a medium containing $51.9 g/L^{-1}$ of fructose and $4.9 g/L^{-1}$ of yeast extract. To prepare sugar solution containing $51.9 g/L^{-1}$ of reducing sugars, mostly fructose plus some glucose, and $1.2 g/L^{-1}$ of total free amino acids which is equivalent concentration of yeast extract, $76.6 g/L^{-1}$ of JA should be hydrolyzed. Glucose was found to be equivalent to fructose in terms of a cell growth and lipid production. The supplement of lipid-extracted Aurantiochytrium (LEA) hydrolysate to the JA hydrolysate resulted in increased biomass production supplying additional minerals including calcium. This indicated that carbon, nitrogen, and minerals for Aurantiochytrium sp. based DHA production could be supplied by JA as the sole substrate with the recycle of LEA after hydrolysis. Such recycle of LEA would lead to the development of Aurantiochytrium sp. cultivation process with no or minimum waste generation. A raw material cost analysis revealed that up to 50% of material cost could be reduced by replacing glucose medium with JA hydrolysate. Taken together, these results suggest that the utilization of JA and recycle of LEA can be a promising alternative for eco-friendly and economical microalgae-based DHA production.
Advisors
Chang, Yong Keunresearcher장용근researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2017.2,[viii, 65 p. :]

Keywords

Aurantiochytrium▼ajerusalem artichoke▼aDHA▼aresponse surface methodology▼aacid hydrolysis▼asulfuric acid▼arecycle▼alipid-extracted biomass; 오란티오키트리움▼a돼지감자▼aDHA▼a반응표면법▼a산 가수분해▼a황산▼a재활용▼a지질이 추출된 바이오매스

URI
http://hdl.handle.net/10203/264924
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=866955&flag=dissertation
Appears in Collection
CBE-Theses_Ph.D.(박사논문)
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