Enhancement of biomass and lipid productivity in ettlia sp. by interaction with wastewater indigenous bacteria = 폐수 내 박테리아와의 상호작용을 통한 에틀리아 바이오매스 및 지질 생산성 증대 연구

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For commercialization of biofuel from microalgae, economical obstacles in cultivation process must be overcome due to its extensive cost. In this study, mixture of swine and municipal wastewater (m-WW) was utilized as a substrate for cultivating Ettlia sp. YC001, one of the most robust microalgal species. Ettlia sp. YC001 grown in the m-WW showed higher pigments and biomass concentrations than ones grown in a conventional TAP medium, along with 25 % higher lipid productivity. In addition, during the cultivation of Ettlia sp. YC001, nutrients in m-WW, such as ammonium and phosphate, which are usually accounted as pollutants, were consumed about 90 % from initial amount. These results suggest that Ettlia sp. YC001 cultivation is possible in the wastewater, also achieving effective removal of pollutants at the same time. According to the m-WW cultivation results, we could find higher biomass productivity though initial nutrient concentration was similar to TAP medium. To investigate the clue of that, we focused on interaction between indigenous bacteria and Ettlia. Among thousands of bacteria existed in the wastewater, some of them have ability stimulate growth of photosynthetic organisms. There are many previous studies using plant growth promoting bacteria (PGPB) to promote the plant growth, along with successful commercialization. Microalgae, which are also photosynthetic organism similar to plants, may also be positively affected to these PGPB to enhance biomass productivity. With pyrosequencing-based analysis during cultivation in m-WW, dominant bacterial species was identified as Pedobacter sp.. To confirm the effect of co-cultivation, Pedobacter sp. was isolated. With co-cultivation with Ettlia sp. YC001, cell division stimulating effect of Pedobacter on Ettlia sp. YC001 was detected and biomass yield was also higher than pure Ettlia culture. Taken together, utilizing wastewater to substitute a conventional medium can be one of the solutions. Furthermore, wastewater indigenous bacteria have synergetic interaction with microalgae.
Advisors
Chang, Yong Keunresearcher장용근researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2018.2,[v, 87 p. :]

Keywords

Microalgae▼aEttlia sp. YC001▼aWastewater treatment▼aPlant growth-promoting bacteria (PGPB)▼aPyrosequencing▼aPedobacter▼aCo-cultivation; 미세조류▼a에틀리아 YC001▼a폐수처리▼a식물성장촉진 박테리아▼a파이로시퀀싱▼a페도박터▼a공배양

URI
http://hdl.handle.net/10203/264909
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=734330&flag=dissertation
Appears in Collection
CBE-Theses_Ph.D.(박사논문)
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