Deciphering yeast physiology using genome-scale analysis for industrial applications = 산업적 응용을 위한 게놈 수준의 효모 생리 분석

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Microbial systems have gained increased importance in biotechnology as platforms for a wide range of applications, including but not limited to biomedical applications, cell factories, and bioremediation. To date, bacterial systems have led the way due to their simplicity of their biological network compared to eukaryotic systems. Furthermore, the wide range of genetic manipulation tools and fast generation times allows for a rapid turnover between hypothesis and experimental validation. However, because of their simplicity, bacterial hosts are often restricted to a specified range of environmental stress. Eukaryotic systems, on the other hand, have a more complex biological network that allows them to adapt more readily to higher levels of stress compared to bacterial systems. Here the physiology of three yeast species, Pichia pastoris, Schizosaccharomyces pombe, and Kluyveromyces marxianus, are investigated through their metabolic network and analyzed using the reconstructed metabolic model of the respective species. In P. pastoris, the physiology of the yeast while producing heterologous proteins was investigated. S. pombe was validated extensively using the single gene knockout library and refined iteratively, resulting in an increased accuracy in predicting mutant phenotype. Finally, K. marxianus physiology was explored to develop systems metabolic engineering strategies for achieving high levels of an industrially important compound, 3-hydroxypropionate. Furthermore, three different versions of the K. marxianus genome-scale metabolic model with changes to charge and mass balance for the reactions to represent the metabolism at three different pH levels.
Advisors
Lee, SangYupresearcher이상엽researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2012
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2012.8,[vi, 230 p. :]

Keywords

Genome-scale metaoblic model▼ayeast▼aschizosaccharomyces pombe▼apichia pastoris▼akluyveromyces marxianus▼aconstraints-based flux analysis; 게놈수준의 대사 모델▼a효모▼a대사흐름분석

URI
http://hdl.handle.net/10203/264875
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=848959&flag=dissertation
Appears in Collection
CBE-Theses_Ph.D.(박사논문)
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