Development of fluorescence-based enzyme activity detection technologies = 형광 기반 효소 활성 검출 기술 개발

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Enzymes as a biocatalyst, play important roles in biological processes including DNA replication, DNA recombination, and transcription and in the fields of biotechnology including polymerase chain reaction, molecular cloning, and DNA sequencing. Their altered levels have served as an indicator for a number of pathological conditions and recognized as attractive therapeutic targets for the treatment of diseases such as cancers and AIDS. Due to their biological and medical significance, a wide variety of detection strategies have been developed for the enzyme activities. In particular, the fluorescent strategies have garnered the special attention due to their advantageous features including high sensitivity, reliability, and simplicity. However, there still remain the drawbacks to be overcome including high-cost and complexity coming from the labeling with fluorophore and quencher and complicated detection procedures. In addition, the sensitivity needs to be further improved. In this dissertation, we have developed novel fluorescent sensing strategies for the enzyme activities by employing unique features of fluorescent nucleobase analogs, nanocomposites, and isothermal amplification methods. Based on this, the activities of base excision repair (BER) enzymes, nucleases, and phosphatases were successfully analyzed, overcoming the above-mentioned limitations of previous methods.
Advisors
Park, Hyun Gyuresearcher박현규researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2019
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2019.2,[xiv, 173 p. :]

Keywords

enzymes▼afluorescent detection technologies▼afluorescence nucleobase analogs▼ananocomposites▼aisothermal amplification methods▼abase excision repair enzymes▼anucleases▼aphosphatases; 효소▼a형광 분석 기술▼a형광염기유사체▼a나노복합체▼a등온핵산증폭 기술▼a염기절단수리 효소▼a핵산분해효소▼a인산기분해효소

URI
http://hdl.handle.net/10203/264864
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=842115&flag=dissertation
Appears in Collection
CBE-Theses_Ph.D.(박사논문)
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