Research on constructions and applications of the engineered antibodies = 변형된 항체의 생산과 응용에 대한 연구

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Antibody is Y-shaped glycoprotein composed of several immunoglobulin domains and plays a critical role in humoral immune response to identify and neutralize foreign antigens. The ability of antibody to bind highly specific is useful in various fields, not only therapeutic but also bioengineering. To overcome drawbacks of antibody, researchers developed antibody variants including Fab, Fv and diabody and discovered new antibodies from other species such as camelid. In spite of their efforts, there still have been limitations. We studied to overcome the limitations and improve their advantages. To enhance utility of diabody, we determined 10 crystal structures of diabodies and revealed that diabody had flexible interface between Fv domains and introduction of disulfide bond made the interface rigid. Furthermore, to increase valence over 2 for enhancing functional affinity, we constructed multivalent scaffold and confirmed that increase of valence enhance functional affinity and multivalent scaffold could distinguish between fibril and monomer by ELISA. These engineered antibodies may have broad applications in numerous fields including the construction of protein nano-assemblies, protein crystallization, the single particle analysis of protein cryo-EM, and the diagnosis for amyloid fibril.
Advisors
Lee, Jie-Ohresearcher이지오researcher
Description
한국과학기술원 :나노과학기술대학원,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 나노과학기술대학원, 2018.2,[v, 91 p. :]

Keywords

Engineered antibody▼adiabody▼adisulfide bond▼amultivalent scaffold▼aamyloid fibril; 변형된 항체▼a다이아바디▼a이황화 결합▼a다가적 지지체▼a아밀로이드 피브릴

URI
http://hdl.handle.net/10203/264608
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=867878&flag=dissertation
Appears in Collection
NT-Theses_Ph.D.(박사논문)
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