DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Han, Jong-In | - |
dc.contributor.advisor | 한종인 | - |
dc.contributor.advisor | Sohn, Jung-Hoon | - |
dc.contributor.advisor | 손정훈 | - |
dc.contributor.author | Park, Hyun Joo | - |
dc.date.accessioned | 2019-08-22T02:37:10Z | - |
dc.date.available | 2019-08-22T02:37:10Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=827728&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/264412 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 건설및환경공학과, 2018.8,[viii, 95 p. :] | - |
dc.description.abstract | Lactic acid is a platform chemical for the sustainable production of various materials. To develop a robust yeast platform for low-pH production of D-lactic acid, an acid-tolerant yeast strain was isolated from grape skins and named Pichia kudriavzevii NG7 by ribosomal RNA sequencing. This strain was able to grow at pH 2.0 and 50°C. For the commercial application of P. kudriavzevii NG7 as a lactic acid producer, the ethanol fermentation pathway was redirected to lactic acid by replacing pyruvate decarboxylase 1 gene (PDC1) with D-lactate dehydrogenase gene (D-LDH) derived from Lactobacillus plantarum. To enhance lactic acid tolerance, this engineered strain was adapted to high lactic acid concentrations, and a new transcriptional regulator, PAR1, responsible for acid tolerance, was identified by whole-genome resequencing. The final engineered strain produced 135 g/L and 154 g/L of D-lactic acid with productivity over 3.66 g/L/h at pH 3.6 and 4.16 g/L/h at pH 4.7, respectively. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Lactic acid | - |
dc.subject | Pichia kudriavzevii | - |
dc.subject | Acid-tolerance | - |
dc.subject | Adaptive evolution | - |
dc.subject | D-젖산▼a내산성 효모▼apichia kudriavzeviiNG7 | - |
dc.subject | 산성발효 | - |
dc.title | (A) study on mass production of lactic acid using acid-tolerance yeast | - |
dc.title.alternative | 내산성 효모를 이용한 젖산의 대량생산 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :건설및환경공학과, | - |
dc.contributor.alternativeauthor | 박현주 | - |
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