(A) study on mass production of lactic acid using acid-tolerance yeast내산성 효모를 이용한 젖산의 대량생산 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 517
  • Download : 0
DC FieldValueLanguage
dc.contributor.advisorHan, Jong-In-
dc.contributor.advisor한종인-
dc.contributor.advisorSohn, Jung-Hoon-
dc.contributor.advisor손정훈-
dc.contributor.authorPark, Hyun Joo-
dc.date.accessioned2019-08-22T02:37:10Z-
dc.date.available2019-08-22T02:37:10Z-
dc.date.issued2018-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=827728&flag=dissertationen_US
dc.identifier.urihttp://hdl.handle.net/10203/264412-
dc.description학위논문(박사) - 한국과학기술원 : 건설및환경공학과, 2018.8,[viii, 95 p. :]-
dc.description.abstractLactic acid is a platform chemical for the sustainable production of various materials. To develop a robust yeast platform for low-pH production of D-lactic acid, an acid-tolerant yeast strain was isolated from grape skins and named Pichia kudriavzevii NG7 by ribosomal RNA sequencing. This strain was able to grow at pH 2.0 and 50°C. For the commercial application of P. kudriavzevii NG7 as a lactic acid producer, the ethanol fermentation pathway was redirected to lactic acid by replacing pyruvate decarboxylase 1 gene (PDC1) with D-lactate dehydrogenase gene (D-LDH) derived from Lactobacillus plantarum. To enhance lactic acid tolerance, this engineered strain was adapted to high lactic acid concentrations, and a new transcriptional regulator, PAR1, responsible for acid tolerance, was identified by whole-genome resequencing. The final engineered strain produced 135 g/L and 154 g/L of D-lactic acid with productivity over 3.66 g/L/h at pH 3.6 and 4.16 g/L/h at pH 4.7, respectively.-
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectLactic acid-
dc.subjectPichia kudriavzevii-
dc.subjectAcid-tolerance-
dc.subjectAdaptive evolution-
dc.subjectD-젖산▼a내산성 효모▼apichia kudriavzeviiNG7-
dc.subject산성발효-
dc.title(A) study on mass production of lactic acid using acid-tolerance yeast-
dc.title.alternative내산성 효모를 이용한 젖산의 대량생산 연구-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN325007-
dc.description.department한국과학기술원 :건설및환경공학과,-
dc.contributor.alternativeauthor박현주-
Appears in Collection
CE-Theses_Ph.D.(박사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0