(A) study on mass production of lactic acid using acid-tolerance yeast = 내산성 효모를 이용한 젖산의 대량생산 연구

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Lactic acid is a platform chemical for the sustainable production of various materials. To develop a robust yeast platform for low-pH production of D-lactic acid, an acid-tolerant yeast strain was isolated from grape skins and named Pichia kudriavzevii NG7 by ribosomal RNA sequencing. This strain was able to grow at pH 2.0 and 50°C. For the commercial application of P. kudriavzevii NG7 as a lactic acid producer, the ethanol fermentation pathway was redirected to lactic acid by replacing pyruvate decarboxylase 1 gene (PDC1) with D-lactate dehydrogenase gene (D-LDH) derived from Lactobacillus plantarum. To enhance lactic acid tolerance, this engineered strain was adapted to high lactic acid concentrations, and a new transcriptional regulator, PAR1, responsible for acid tolerance, was identified by whole-genome resequencing. The final engineered strain produced 135 g/L and 154 g/L of D-lactic acid with productivity over 3.66 g/L/h at pH 3.6 and 4.16 g/L/h at pH 4.7, respectively.
Advisors
Han, Jong-Inresearcher한종인researcherSohn, Jung-Hoonresearcher손정훈researcher
Description
한국과학기술원 :건설및환경공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 건설및환경공학과, 2018.8,[viii, 95 p. :]

Keywords

Lactic acid; Pichia kudriavzevii; Acid-tolerance; Adaptive evolution; D-젖산▼a내산성 효모▼apichia kudriavzeviiNG7; 산성발효

URI
http://hdl.handle.net/10203/264412
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=827728&flag=dissertation
Appears in Collection
CE-Theses_Ph.D.(박사논문)
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