PRODUCTION OF BACILLUS-THURINGIENSIS SPORES IN TOTAL CELL RETENTION CULTURE AND 2-STAGE CONTINUOUS-CULTURE USING AN INTERNAL CERAMIC FILTER SYSTEM

Cited 22 time in webofscience Cited 0 time in scopus
  • Hit : 26
  • Download : 0
The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial cell bleeding. In the TCRC, fed by 50 g/L of glucose, a spore concentration of 1.6 x 10(10) CFU/mL was obtained with a spore percentage of greater than 95% and a maximum cell mass of 82.2 g/L. The volumetric productivity was four times higher than that obtained from batch cultivation. In the two-stage continuous culture with partial cell bleeding spore concentration was strongly dependent on the bleed ratio. The spore concentration of 1.8 x 10(9) CFU/mL and the spore percentage of 70% were obtained at the second stage when a bleed ratio of 0.33 and a dilution rate of 0.23 h-1 were used. (C) 1993 John Wiley & Sons, Inc.
Publisher
WILEY
Issue Date
1993-11
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.42, no.9, pp.1107 - 1112

ISSN
0006-3592
DOI
10.1002/bit.260420913
URI
http://hdl.handle.net/10203/262852
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 22 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0