Glutamine substitution: the role it can play to enhance therapeutic protein production

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 14
  • Download : 0
The biopharmaceutical market is driven by the steady increase in demand for therapeutic proteins produced in mammalian cells. Glutamine is a main nitrogen source and also a main energy source with glucose in mammalian cell cultures for therapeutic protein production. As a result of glutamine metabolism and the natural decomposition of glutamine, ammonia, which is known to negatively affect cell growth, protein production and sialylation of recombinant glycoprotein, is necessarily accumulated in a culture medium. This review highlights the current strategies and achievements in overcoming the negative effect of ammonia through the glutamine substitution by less ammoniagenic substrates, such as glutamate, pyruvate and a-ketoglutarate.
Publisher
OPEN ACCESS JOURNALS
Issue Date
2015
Language
English
Article Type
Review
Citation

PHARMACEUTICAL BIOPROCESSING, v.3, no.3, pp.249 - 261

ISSN
2048-9145
DOI
10.4155/PBP.15.6
URI
http://hdl.handle.net/10203/261693
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0