Method and kit for analyzing protein-protein interaction using nanocluster formation

Abstract

For efficient analysis of a protein-protein interaction, the present disclosure provides a kit for analyzing a protein-protein interaction, the kit including: a 1st expression vector including a 1st polynucleotide and a multi-cloning site, wherein, the 1st polynucleotide is operably linked to a promoter and encodes a 1st fusion protein having a 1st fluorescence protein and a 1st self-assembly protein, and the multi-cloning site is a site where a polynucleotide encoding a bait protein may be operably linked to the polynucleotide encoding the 1st fusion protein; and a 2nd expression vector including a 2nd polynucleotide and a multi-cloning site, wherein, the 2nd polynucleotide is operably linked to a promoter and encodes a 2nd fusion protein having a 2nd fluorescence protein and a 2nd self-assembly protein, and the multi-cloning site is a site where a polynucleotide encoding a prey protein may be operably linked to the polynucleotide encoding the 2nd fusion protein.