Hexagonally packed microwell plates for hypoxic microenvironment induction in tumorspheres

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The growth of a solid tumor in a confined microenvironment leads to increased compression, hypoxic/nutrition gradient within the tumor. However, Tumorspheres (TSs) cultured in free of spatial confinement do not exhibit physiological compressive stress. Here, we designed a microwell-based sphero-plate that allowed TSs to experience spatial confinement. On this platform, TSs underwent growth-mediated compressive stress and formed hypoxic cores. Moreover, drug resistance was enhanced by the hypoxia-inducible signaling and decreased by HIF-1 targeted drug within compact TSs. These findings highlight the impact of the design of the TSs-based drug testing platform.
Publisher
ELSEVIER SCIENCE INC
Issue Date
2019-04
Language
English
Article Type
Article
Citation

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.72, pp.67 - 72

ISSN
1226-086X
DOI
10.1016/j.jiec.2018.12.010
URI
http://hdl.handle.net/10203/251985
Appears in Collection
BiS-Journal Papers(저널논문)
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