DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ihee, Hyotcherl | ko |
dc.contributor.author | Rajagopal, S | ko |
dc.contributor.author | Srajer, V | ko |
dc.contributor.author | Pahl, R | ko |
dc.contributor.author | Anderson, S | ko |
dc.contributor.author | Schmidt, M | ko |
dc.contributor.author | Schotte, F | ko |
dc.contributor.author | Anfinrud, PA | ko |
dc.contributor.author | Wulff, M | ko |
dc.contributor.author | Moffat, K | ko |
dc.date.accessioned | 2019-03-07T04:16:00Z | - |
dc.date.available | 2019-03-07T04:16:00Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-05 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.102, no.20, pp.7145 - 7150 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250827 | - |
dc.description.abstract | Determining 3D intermediate structures during the biological action of proteins in real time under ambient conditions is essential for understanding how proteins function. Here we use timer-esolved Laue crystallography to extract short-lived intermediate structures and thereby unveil signal transduction in the blue light photoreceptor photoactive yellow protein (PYP) from Halorhodospira halophila. By analyzing a comprehensive set of Laue data during the PYP photocycle (forty-seven time points from one nanosecond to one second), we track all atoms in PYP during its photocycle and directly observe how absorption of a blue light photon by its p-coumaric acid chromophore triggers a reversible photocycle. We identify a complex chemical mechanism characterized by five distinct structural intermediates. Structural changes at the chromophore in the early, red-shifted intermediates are transduced to the exterior of the protein in the late, blue-shifted intermediates through an initial "volume-conserving" isomerization of the chromophore and the progressive disruption of hydrogen bonds between the chromophore and its surrounding binding pocket. These results yield a comprehensive view of the PYP photocycle when seen in the light of previous biophysical studies on the system. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | P-COUMARIC ACID | - |
dc.subject | ECTOTHIORHODOSPIRA-HALOPHILA | - |
dc.subject | PHOTORECEPTOR PROTEIN | - |
dc.subject | PHOTOCYCLE INTERMEDIATE | - |
dc.subject | PHOTOTROPHIC BACTERIUM | - |
dc.subject | ANGSTROM RESOLUTION | - |
dc.subject | STRUCTURAL-CHANGES | - |
dc.subject | E46Q MUTANT | - |
dc.subject | CHROMOPHORE | - |
dc.subject | KINETICS | - |
dc.title | Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds | - |
dc.type | Article | - |
dc.identifier.wosid | 000229292200018 | - |
dc.identifier.scopusid | 2-s2.0-21044432995 | - |
dc.type.rims | ART | - |
dc.citation.volume | 102 | - |
dc.citation.issue | 20 | - |
dc.citation.beginningpage | 7145 | - |
dc.citation.endingpage | 7150 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.0409035102 | - |
dc.contributor.localauthor | Ihee, Hyotcherl | - |
dc.contributor.nonIdAuthor | Rajagopal, S | - |
dc.contributor.nonIdAuthor | Srajer, V | - |
dc.contributor.nonIdAuthor | Pahl, R | - |
dc.contributor.nonIdAuthor | Anderson, S | - |
dc.contributor.nonIdAuthor | Schmidt, M | - |
dc.contributor.nonIdAuthor | Schotte, F | - |
dc.contributor.nonIdAuthor | Anfinrud, PA | - |
dc.contributor.nonIdAuthor | Wulff, M | - |
dc.contributor.nonIdAuthor | Moffat, K | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | intermediates | - |
dc.subject.keywordAuthor | mechanism | - |
dc.subject.keywordAuthor | signal transduction | - |
dc.subject.keywordAuthor | time-resolved crystallography | - |
dc.subject.keywordAuthor | singular value decomposition | - |
dc.subject.keywordPlus | P-COUMARIC ACID | - |
dc.subject.keywordPlus | ECTOTHIORHODOSPIRA-HALOPHILA | - |
dc.subject.keywordPlus | PHOTORECEPTOR PROTEIN | - |
dc.subject.keywordPlus | PHOTOCYCLE INTERMEDIATE | - |
dc.subject.keywordPlus | PHOTOTROPHIC BACTERIUM | - |
dc.subject.keywordPlus | ANGSTROM RESOLUTION | - |
dc.subject.keywordPlus | STRUCTURAL-CHANGES | - |
dc.subject.keywordPlus | E46Q MUTANT | - |
dc.subject.keywordPlus | CHROMOPHORE | - |
dc.subject.keywordPlus | KINETICS | - |
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