Slow-Wave Recordings From Micro-Sized Neural Clusters Using Multiwell Type Microelectrode Arrays

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Objective: The use of microelectrode array (MEA) recordings is a very effective neurophysiological method because it is able to continuously and noninvasively obtain the spatiotemporal information of electrical activity from many neurons constituting a neural network. Very recently, studies have been published that used MEAs for the measurement of a low-frequency component of electrical activity as an indicator of diverse activity of cultured neurons. The occurrence of low-frequency activities has electrophysiological information that does not include the information from fast spikes. However, there is no in vitro experimental model suitable for measuring the low-frequency activities (slow-waves) for further study. Methods: Neural clusters consisting of dozens of neurons were placed directly onto each electrode of an MEA from which fast spikes and slow-waves were measured. Results: We obtained sufficient data on the early development patterns of the slow-waves and the spikes measured from many independent neural clusters confirming that the slow-waves occurred first before the emergence of the spikes in the neural clusters. We also showed that changes in the occurrence frequency of the slow-waves for synaptic blockers were measured from a large number of independent cultures. Conclusion: Microsized neural cluster arrays, which can be combined with conventional MEAs, are suitable for multiple simultaneous recordings of slow-waves. Significance: Our technology provides a simple but useful method to study the generation of a low-frequency component of the electrical activity in cultured neural networks that are not yet well known as well as to expand the use of conventional MEAs.
Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Issue Date
2019-02
Language
English
Article Type
Article
Citation

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, v.66, no.2, pp.403 - 410

ISSN
0018-9294
DOI
10.1109/TBME.2018.2843793
URI
http://hdl.handle.net/10203/250351
Appears in Collection
BiS-Journal Papers(저널논문)
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