PKC phosphorylation disrupts gap juctional communication at Go/S phase in clone 9 cells.

Gap junctional communication during the progression of cell cycle from quiescent G0 to S phase was examined in cultured clone 9 rat liver cells. The transfer of scrape-loaded fluorescent dye was suppressed immediately after the stimulation of cell cycle progression in a synchronized cell population. Northern blot analysis showed that the temporal disturbance of gap junctional communication in cells passing from G0 to S phase did not result from transcriptional down-regulation of connexin 43. It was also found that the PKC inhibitor, calphostin C, was able to restore intercellular communication in serum stimulated cells. Data suggest a control mechanism by PKC mediated phosphorylation in the regulation of gap junction function which is vulnerable to cell cycling. The loss of gap junctional communication correlated with the increased phosphorylation of connexin 43 on serine residues in clone 9 cells
Publisher
Springer Verlag
Issue Date
1997-02
Keywords

connexin 43; cell cycle; phosphorylation; protein kinase C

Citation

Molecular and Cellular Biochemistry, Vol.167, pp.41-49

ISSN
0300-8177
URI
http://hdl.handle.net/10203/25016
Appears in Collection
BS-Conference Papers(학술회의논문)
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