DC Field | Value | Language |
---|---|---|
dc.contributor.author | Halle, Mahesh B. | ko |
dc.contributor.author | Lee, KyungJin | ko |
dc.contributor.author | Yudhistira, Tesla | ko |
dc.contributor.author | Choi, Jaehyuck | ko |
dc.contributor.author | Park, Hee-Sung | ko |
dc.contributor.author | Churchill, David G | ko |
dc.date.accessioned | 2019-01-23T06:19:43Z | - |
dc.date.available | 2019-01-23T06:19:43Z | - |
dc.date.created | 2018-10-17 | - |
dc.date.issued | 2018-11 | - |
dc.identifier.citation | CHEMISTRY-AN ASIAN JOURNAL, v.13, no.24, pp.3895 - 3902 | - |
dc.identifier.issn | 1861-4728 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250020 | - |
dc.description.abstract | A simple one‐step synthesis of fluorescent probe HemiSe has been developed for the detection of superoxide (O2.−). The probe undergoes reaction specifically with O2.− when in the presence of other competitive ROS/RNS/metal ions. The diphenylselenide was incorporated to completely quench the fluorescence of the hemicyanine unit through the action of a photoinduced electron transfer (PET) photomechanism. However, after the addition of O2.−, the latent fluorophore regains its fluorescence owing to the reaction at the C=C bond of the hemicyanine with O2.− through nucleophilic attack; the increase in blue emission is due to a reaction of the double bond within HemiSe followed by an increase in fluorescence quantum yield (Φ) up to 0.45; the limit of detection (LOD) is 11.9 nm. A time‐dependent study shows that HemiSe can detect superoxide within 13 min with high sensitivity, high selectivity, over a wide pH range, and through confirmation with a xanthine/xanthine oxidase biochemical assay (λem=439 nm). A study in the RAW 264.7 macrophage living cells also shows that HemiSe is not toxic, cell permeable (experimental log P=2.11); confocal imaging results show that HemiSe can detect O2.− in endogenous and exogeneous systems. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | A hemicyanine-embedded diphenylselenide-containing probe “HemiSe” in which SePh2 stays reduced for selective detection of superoxide in living cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000453745000014 | - |
dc.identifier.scopusid | 2-s2.0-85056458051 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 24 | - |
dc.citation.beginningpage | 3895 | - |
dc.citation.endingpage | 3902 | - |
dc.citation.publicationname | CHEMISTRY-AN ASIAN JOURNAL | - |
dc.identifier.doi | 10.1002/asia.201801339 | - |
dc.contributor.localauthor | Park, Hee-Sung | - |
dc.contributor.localauthor | Churchill, David G | - |
dc.contributor.nonIdAuthor | Halle, Mahesh B. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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