Design and Fabrication of Miniaturized Neuronal Circuits on Microelectrode Arrays Using Agarose Hydrogel Micro-molding Technique

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Dissociated neuronal cultures combined with planar-type microelectrode arrays (MEAs) have been used as a promising read-out platform for the application of cell-based biosensors. There are increasing interests in engineering neuronal cultures to form the desired network topology by surface micropatterning technology. Here, we report a long-term cultivation of primary hippocampal neurons on microelectrode arrays using soft-lithography. Ordered hippocampal neuronal networks were formed by seeding neurons in agarose-microwells and inducing neurite outgrowth through microgrooves. Unlike previous approaches, our technique allowed us to design networks with various microwells on microelectrode arrays with high repeatability. These hippocampal network chips were cultivated for 30 days with excellent pattern fidelity, and neural spikes were successfully measured. We also found that spontaneous activity of the networks could be enhanced by acute disinhibition of inhibitory synapses. The proposed patterning method for neuronal network chips will be a potentially powerful tool for cell-based drug-screening applications.
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Issue Date
2018-09
Language
English
Article Type
Article
Keywords

CORTICAL-NEURONS; NEURAL-NETWORKS; CULTURES; STIMULATION

Citation

BIOCHIP JOURNAL, v.12, no.3, pp.193 - 201

ISSN
1976-0280
DOI
10.1007/s13206-018-2308-y
URI
http://hdl.handle.net/10203/246039
Appears in Collection
BiS-Journal Papers(저널논문)
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