Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section

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We present here a novel microfluidic platform that can perform microfluidic on-chip immunohistochemistry (IHC) processes on a formalin-fixed paraffin-embedded section slide. Unlike previous microfluidic IHC studies, our microfluidic chip made of organic solvent-resistant polyurethane acrylate (PUA) is capable of conducting on-chip IHC processes consecutively. A narrow channel wall structure of the PUA chip shows effective sealing by pressure-based reversible assembly with a section slide. We performed both on-chip IHC and conventional IHC processes and compared the IHC results based on the immunostaining intensity. The result showed that the effects of the on-chip deparaffinization, antigen retrieval, and immunoreaction processes on the IHC result were equivalent to conventional methods while reducing the total process time to less than 1/2. The experiment with breast cancer tissue shows that human epidermal growth factor receptor 2 (HER2) classification can be performed by obtaining a clearly distinguishable immunostaining intensity according to the HER2 expression level. We expect our on-chip microfluidic platform to provide a facile technique suitable for miniaturized, automated, and precise diagnostic devices, including a point-of-care device. Published by AIP Publishing.
Publisher
AMER INST PHYSICS
Issue Date
2018-07
Language
English
Article Type
Article; Proceedings Paper
Keywords

BREAST-CANCER; ANTIGEN RETRIEVAL; BIOMARKERS; RECEPTOR; IMMUNOCYTOCHEMISTRY; CARCINOMAS; EXPRESSION; PLATFORM; CELLS; TOOL

Citation

BIOMICROFLUIDICS, v.12, no.4, pp.044110

ISSN
1932-1058
DOI
10.1063/1.5042347
URI
http://hdl.handle.net/10203/245937
Appears in Collection
BiS-Journal Papers(저널논문)
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