An efficient isolation of foodborne pathogen using surface-modified porous sponge

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Rapid and efficient detection of pathogenic bacteria from food is critical to prevent epidemic food poisoning. However, the isolation of pathogenic bacteria from spoiled food is hampered by the lack of proper cell cultivation and/or isolation methods. Most of currently used methods suffer from complex, time-consuming culturing steps, low scalability, and high operation cost. Herein, we developed an alternative approach for the isolation of pathogenic bacteria directly from food using a surface-modified, highly porous sponge via initiated chemical vapor deposition (iCVD) process. A hydrophobic polymer, poly(2,4,6,8-tetravinyl-2,4,6,8-tetramethyl cyclotetrasiloxane) (pV4D4), was deposited conformally on amphiphilic 3-dimensional (3D) melamine sponge to incorporate hydrophobicity as well as oleophilicity to the porous sponge surface, which is appropriate for absorbing oil component selectively from food extracts. Furthermore, the surface-modified sponge was capable of the isolation of Escherichia coli O157:H7 (E. coli O157:H7) from heterogeneous mixture with oil/water/food particles with undistinguisible efficiency compare to artificial model system. The surface-modified sponge developed in this study will be a novel platform for oil/water separation and isolation of foodborne pathogens directly from heterogeneous mixture to enhance the efficiency of molecular diagnostics.
Publisher
ELSEVIER SCI LTD
Issue Date
2019-01
Language
English
Article Type
Article
Keywords

ESCHERICHIA-COLI O157H7; IN-WATER EMULSIONS; OIL/WATER SEPARATION; RAPID DETECTION; FOOD; OIL; PCR; FABRICATION; ASSAYS; NANOPARTICLES

Citation

FOOD CHEMISTRY, v.270, pp.445 - 451

ISSN
0308-8146
DOI
10.1016/j.foodchem.2018.07.125
URI
http://hdl.handle.net/10203/245861
Appears in Collection
CBE-Journal Papers(저널논문)
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