Biosynthesis of (R)-3-hydroxyalkanoic acids by metabolically engineered Escherichia coli

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An efficient system for the production of (R)-hydroxyalkanoic acids (RHAs) was developed in natural polyhydroxyalkanoate (PHA)-producing bacteria and recombinant Escherichia coli. Acidic alcoholysis of purified PHA and in vivo depolymerization of PHA accumulated in the cells allowed the production of RHAs. In recombinant E. coli, RHA production was achieved by removing CoA from (R)-3-hydroxyacyl-CoA and by in vivo depolymerization of PHA. When the recombinant E. coli harboring the Ralstonia eutropha PHA biosynthesis genes and the depolymerase gene was cultured in a complex or a chemically defined medium containing glucose, (R)-3-hydroxybutyric acid (R3HB) was produced as monomers and dimers. R3HB dimers could be efficiently converted to monomers by mild alkaline heat treatment. A stable recombinant E. coli strain in which the R. eutropha PHA biosynthesis genes were integrated into the chromosome disrupting the pta gene was constructed and examined for the production of R3HB. When the R. eutropha intracellular depolymerase gene was expressed by using a stable plasmid containing the hok/sok locus of plasmid R1, R3HB could be efficiently produced.
Publisher
HUMANA PRESS INC
Issue Date
2004-03
Language
English
Article Type
Article; Proceedings Paper
Keywords

ALCALIGENES-EUTROPHUS H16; BACTERIAL POLYHYDROXYALKANOATES; CLONING; PHB; (R)-3-HYDROXYBUTANOATE; REDUCTION; EFFICIENT; CHEMICALS; SYSTEM; YEAST

Citation

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.113, pp.373 - 379

ISSN
0273-2289
URI
http://hdl.handle.net/10203/24556
Appears in Collection
CBE-Journal Papers(저널논문)
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