Ureolytic/Non-ureolytic bacteria co-cultured self-healing agent for cementitious materials crack repair

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The present study investigated the CaCO3 precipitation performance of ureolytic and non-ureolytic bacteria co-cultured as a self-healing agent for cementitious materials crack repair. Three different inoculum ratios of ureolytic Sporosarcina pasteurii and non-ureolytic Bacillus thuringiensis (10:0, 8:2, or 5:5) were used. The effect of coculturing ureolytic and non-ureolytic bacteria on microbial metabolism was investigated by measuring the rate of growth in urea-containing medium and the rate of NH4 + and CaCO3 production in urea-calcium lactate medium. The self-healing efficiency of co-cultured bacteria was examined by exposing cement mortar specimens with predefined cracks to media containing single urease-producing or co-cultured bacteria. The obtained results provide new findings, where CaCO3 precipitation is improved by co-culturing ureolytic and non-ureolytic bacteria, owing to the relatively faster growth rate of non-ureolytic bacteria. The crack filling rate correlated with the width of crack, in particular, specimens with a smaller crack width showed the faster filling effect, indicating that the crack width can be a dominant factor influencing the CaCO3 precipitation capacity of co-cultured bacteria.
Publisher
MDPI AG
Issue Date
2018-05
Language
English
Article Type
Article
Citation

MATERIALS, v.11, no.5

ISSN
1996-1944
DOI
10.3390/ma11050782
URI
http://hdl.handle.net/10203/244065
Appears in Collection
CE-Journal Papers(저널논문)
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