Transcriptomic approach for the identification of novel regulators involved in drosophila olfaction = 초파리 후각에 관련된 새로운 조절자들의 동정을 위한 전사체적 접근

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As insect olfaction is closely related to human society in many ways, it is of great interest to find its underlying process. Insects detect odorants through odorant receptors (ORs) that form channels with the common olfactory co-receptor (Orco) that is conserved among insects including fruit fly Drosophila melanogaster and is critical for the olfactory function. Although many aspects of insect olfaction have been revealed, it is not yet clear how exactly Orco is expressed and which genes in particular are related exclusively to the process. In this study, we generated a transcriptome of olfactory sensory neurons (OSNs) and profiled the changes in the genomic repertoire. By screening several candidates selected from the profile, we elucidated a novel transcription factor, CG7786. We validated CG7786 by checking the function and localization of ORs depending on the expression level of CG7786 and confirmed that it is involved in the expression of several ORs. CG7786 not only plays the critical role in the proper function of ORs under its control, but also is modulates the transcription of those ORs. The loss of OR expression in the CG7786 mutant background could be rescued by OR-specific expression of CG7786 both transcriptionally and functionally. This approach was sufficient to validate the effectiveness of transcriptomic approaches in search of novel genes related to Drosophila olfaction.
Advisors
Jones, Walton D.researcher존스, 월튼researcher
Description
한국과학기술원 :생명과학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2017.2,[iv, 64 p. :]

Keywords

Drosophila melanogaster; odorant receptor; Orco; CG7786; transcriptome; 노랑초파리; 후각수용체; 후각보조수용체; 전사체

URI
http://hdl.handle.net/10203/243045
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=675241&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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