DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Han, Jin-Hee | - |
dc.contributor.advisor | 한진희 | - |
dc.contributor.author | Choi, Kwang-Yeon | - |
dc.contributor.author | 최광연 | - |
dc.date.accessioned | 2018-05-23T19:36:40Z | - |
dc.date.available | 2018-05-23T19:36:40Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=669255&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/241970 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 의과학대학원, 2015.8,[vi, 79 p. :] | - |
dc.description.abstract | Epigenetic regulation of gene expression has been implicated in long-lasting memory formation. However, little is known about the role of chromatin remodeling mechanism in learning and memory. Recent study reports that BAF53b, a post-mitotic neuron-specific subunit of Brg/Brm-associated factor (BAF) chromatin remodeling complex is involved in hippocampus-dependent memory formation. However, whether BAF53b plays a role for long-term fear memory formation in lateral amygdala (LA), and its underlying mechanisms remain unknown. To address these issues, I used viral vectors to manipulate expression level of BAF53b specifically in LA and investigated its effects on long-term memory formation and synaptic structural plasticity using auditory fear conditioning paradigm. First, I found that Baf53b knockdown in LA neurons impaired long-term, but not short-term, fear memory formation, proving that BAF53b function in LA is essential for long-term fear memory formation. Second, transient BAF53b overexpression in LA enhanced long-term memory up to one month with no effect on short-term memory. Third, at the synapse level, confocal imaging of synaptic spines, visualized with GFP or dye injection in LA after fear learning revealed that the density of thin-type spine was specifically increased in BAF53b-overexpressing neurons one hr after learning, while head volume of mushroom-type spine in BAF53b knockdown neurons was significantly decreased compared to that in control neurons 24 hrs after learning. In parallel to this result, RT-PCR analysis showed that Baf53b knockdown blocked activity-dependent upregulation of miR132, which is known to be involved in regulation of activity-dependent spine enlargement. Taken together, my results demonstrate that BAF53b in LA is essential for long-term fear memory formation of auditory fear conditioning and reveal novel underlying mechanisms of BAF53b during long-lasting memory formation. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | BAF53b | - |
dc.subject | epigenetic | - |
dc.subject | nBAF | - |
dc.subject | structural plasticity | - |
dc.subject | miR132 | - |
dc.subject | 구조적 가소성 | - |
dc.subject | 후성유전 | - |
dc.title | (The) role of BAF chromatin remodeling complex in long-term fear memmory formation | - |
dc.title.alternative | 공포 장기기억 형성에서 BAF 크로마틴 리모델링 복합체의 기능 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :의과학대학원, | - |
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