Label-free high-resolution 3-D imaging of gold nanoparticles inside live cells using optical diffraction tomography

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dc.contributor.authorKim, Doyeonko
dc.contributor.authorOh, Nuriko
dc.contributor.authorKim, Kyoohyunko
dc.contributor.authorLee, SangYunko
dc.contributor.authorPack, Chan-Giko
dc.contributor.authorPark, Ji-Hoko
dc.contributor.authorPark, Yong Keunko
dc.date.accessioned2018-05-23T06:31:03Z-
dc.date.available2018-05-23T06:31:03Z-
dc.date.created2017-12-20-
dc.date.created2017-12-20-
dc.date.created2017-12-20-
dc.date.issued2018-03-
dc.identifier.citationMETHODS, v.136, pp.160 - 167-
dc.identifier.issn1046-2023-
dc.identifier.urihttp://hdl.handle.net/10203/241538-
dc.description.abstractDelivery of gold nanoparticles (GNPs) into live cells has high potentials, ranging from molecular-specific imaging, photodiagnostics, to photothermal therapy. However, studying the long-term dynamics of cells with GNPs using conventional fluorescence techniques suffers from phototoxicity and photobleaching. Here, we present a method for 3-D imaging of GNPs inside live cells exploiting refractive index (RI) as imaging contrast. Employing optical diffraction tomography, 3-D RI tomograms of live cells with GNPs are precisely measured for an extended period with sub-micrometer resolution. The locations and contents of GNPs in live cells are precisely addressed and quantified due to their distinctly high RI values, which was validated by confocal fluorescence imaging of fluorescent dye conjugated GNPs. In addition, we perform quantitative imaging analysis including the segmentations of GNPs in the cytosol, the volume distributions of aggregated GNPs, and the temporal evolution of GNPs contents in HeLa and 4T1 cells. (C) 2017 Elsevier Inc. All rights reserved.-
dc.languageEnglish-
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE-
dc.titleLabel-free high-resolution 3-D imaging of gold nanoparticles inside live cells using optical diffraction tomography-
dc.typeArticle-
dc.identifier.wosid000428968500018-
dc.identifier.scopusid2-s2.0-85025100942-
dc.type.rimsART-
dc.citation.volume136-
dc.citation.beginningpage160-
dc.citation.endingpage167-
dc.citation.publicationnameMETHODS-
dc.identifier.doi10.1016/j.ymeth.2017.07.008-
dc.contributor.localauthorPark, Ji-Ho-
dc.contributor.localauthorPark, Yong Keun-
dc.contributor.nonIdAuthorKim, Doyeon-
dc.contributor.nonIdAuthorPack, Chan-Gi-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorLabel-free imaging-
dc.subject.keywordAuthorOptical diffraction tomography-
dc.subject.keywordAuthorGold nanoparticles imaging-
dc.subject.keywordAuthorFast 3D acquisition-
dc.subject.keywordAuthorQuantitative image analysis-
dc.subject.keywordPlusDIGITAL HOLOGRAPHIC MICROSCOPY-
dc.subject.keywordPlusREFRACTIVE-INDEX TOMOGRAPHY-
dc.subject.keywordPlusTRANSFORM LIGHT-SCATTERING-
dc.subject.keywordPlusPHOTOTHERMAL THERAPY-
dc.subject.keywordPlusPROTEIN CORONA-
dc.subject.keywordPlusCANCER-CELLS-
dc.subject.keywordPlusBLOOD-CELLS-
dc.subject.keywordPlusRECONSTRUCTION-
dc.subject.keywordPlusNANORODS-
dc.subject.keywordPlusMAPS-
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BiS-Journal Papers(저널논문)PH-Journal Papers(저널논문)
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