A simple, sensitive, and label-free assay for alkaline phosphatase activity based on target-promoted exponential strand displacement amplification

Cited 13 time in webofscience Cited 0 time in scopus
  • Hit : 550
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorLee, Chang Yeolko
dc.contributor.authorPark, Ki Sooko
dc.contributor.authorPark, Hyun Gyuko
dc.date.accessioned2018-04-24T06:32:07Z-
dc.date.available2018-04-24T06:32:07Z-
dc.date.created2018-04-18-
dc.date.created2018-04-18-
dc.date.created2018-04-18-
dc.date.issued2018-06-
dc.identifier.citationSENSORS AND ACTUATORS B-CHEMICAL, v.262, pp.1001 - 1005-
dc.identifier.issn0925-4005-
dc.identifier.urihttp://hdl.handle.net/10203/241399-
dc.description.abstractWe herein describe a simple, sensitive, and label-free strategy for alkaline phosphatase (ALP) activity based on target-promoted exponential strand displacement amplification (eSDA). The developed system utilizes 3'-phosphorylated DNA probes whose phosphate group at the 3'-end blocks the extension by DNA polymerase. Only in the presence of ALP, the phosphate group at the 3'-end is transformed to the hydroxyl group, which enables the extension by DNA polymerase and produces the double-stranded recognition sequence for nicking endonuclease. Consequently, the effective eSDA is executed, generating a large number of duplex products with the high fluorescence signal from SYBR green I. Based on this novel strategy, we determined the ALP activity down to 0.47 U/L with the high selectivity. In addition, the practical applicability of this assaying system was successfully demonstrated by reliably determining the ALP activity in human serum. (C) 2018 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE SA-
dc.titleA simple, sensitive, and label-free assay for alkaline phosphatase activity based on target-promoted exponential strand displacement amplification-
dc.typeArticle-
dc.identifier.wosid000427460600119-
dc.identifier.scopusid2-s2.0-85042073037-
dc.type.rimsART-
dc.citation.volume262-
dc.citation.beginningpage1001-
dc.citation.endingpage1005-
dc.citation.publicationnameSENSORS AND ACTUATORS B-CHEMICAL-
dc.identifier.doi10.1016/j.snb.2018.02.054-
dc.contributor.localauthorPark, Hyun Gyu-
dc.contributor.nonIdAuthorPark, Ki Soo-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorAlkaline phosphatase-
dc.subject.keywordAuthorDephosphorylation-
dc.subject.keywordAuthorExponential strand displacement amplification-
dc.subject.keywordAuthorLabel-free-
dc.subject.keywordAuthorSYBR green I-
dc.subject.keywordAuthorEnzyme activity assay-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusSERUM-
dc.subject.keywordPlusPYROPHOSPHATE-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusEXONUCLEASE-
dc.subject.keywordPlusBIOSENSOR-
dc.subject.keywordPlusDISEASE-
dc.subject.keywordPlusPROBE-
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 13 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0