Amine-Reactive Poly(pentafluorophenyl acrylate) Brush Platforms for Cleaner Protein Purification

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dc.contributor.authorSon, Hyunjooko
dc.contributor.authorKu, Jayoungko
dc.contributor.authorKim, Yoosikko
dc.contributor.authorLi, Shengko
dc.contributor.authorChar, Kookheonko
dc.date.accessioned2018-04-24T04:26:31Z-
dc.date.available2018-04-24T04:26:31Z-
dc.date.created2018-04-02-
dc.date.created2018-04-02-
dc.date.issued2018-03-
dc.identifier.citationBIOMACROMOLECULES, v.19, no.3, pp.951 - 961-
dc.identifier.issn1525-7797-
dc.identifier.urihttp://hdl.handle.net/10203/241218-
dc.description.abstractReactive pentafluorophenyl acrylate (PFPA) polymer brushes grafted on silica particles were prepared using surface-initiated reversible addition and fragmentation chain transfer polymerization. The polymer brush was successfully immobilized with antibody, then used for protein separation. The immunoprecipitated proteins showed successful enrichment of target protein, with reduced nonspecific background and less contamination from eluted antibodies. To further improve protein recovery, the hydrophobic poly(PFPA) brush was modified with hydrophilic poly(ethylene glycol) (PEG). The partially PEG-substituted poly(PFPA) brush showed better dispersion in aqueous solution, leading to improved antibody immobilization efficiency. By optimizing both the brush molecular weight and the degree of PEG substitution, an optimal balance between surface hydrophilicity and number of available PFP units was found, leading to efficient target protein purification. This study shows that poly(PFPA) platform offers a versatile approach to prepare biomolecule-activated surfaces with tunable surface property, which has potential applications in protein separation and other areas.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.subjectA-ANTIBODY ADSORBENT-
dc.subjectPOLYMER BRUSHES-
dc.subjectREVERSIBLE ADDITION-
dc.subjectRADICAL POLYMERIZATION-
dc.subjectGRAFTING DENSITY-
dc.subjectCROSS-LINKING-
dc.subjectTHIN-FILMS-
dc.subjectIN-VIVO-
dc.subjectIMMOBILIZATION-
dc.subjectSURFACE-
dc.titleAmine-Reactive Poly(pentafluorophenyl acrylate) Brush Platforms for Cleaner Protein Purification-
dc.typeArticle-
dc.identifier.wosid000427539600025-
dc.identifier.scopusid2-s2.0-85043581885-
dc.type.rimsART-
dc.citation.volume19-
dc.citation.issue3-
dc.citation.beginningpage951-
dc.citation.endingpage961-
dc.citation.publicationnameBIOMACROMOLECULES-
dc.identifier.doi10.1021/acs.biomac.7b01736-
dc.contributor.localauthorKim, Yoosik-
dc.contributor.localauthorLi, Sheng-
dc.contributor.nonIdAuthorSon, Hyunjoo-
dc.contributor.nonIdAuthorKu, Jayoung-
dc.contributor.nonIdAuthorChar, Kookheon-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusA-ANTIBODY ADSORBENT-
dc.subject.keywordPlusPOLYMER BRUSHES-
dc.subject.keywordPlusREVERSIBLE ADDITION-
dc.subject.keywordPlusRADICAL POLYMERIZATION-
dc.subject.keywordPlusGRAFTING DENSITY-
dc.subject.keywordPlusCROSS-LINKING-
dc.subject.keywordPlusTHIN-FILMS-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusIMMOBILIZATION-
dc.subject.keywordPlusSURFACE-
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