Amine-Reactive Poly(pentafluorophenyl acrylate) Brush Platforms for Cleaner Protein Purification

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Reactive pentafluorophenyl acrylate (PFPA) polymer brushes grafted on silica particles were prepared using surface-initiated reversible addition and fragmentation chain transfer polymerization. The polymer brush was successfully immobilized with antibody, then used for protein separation. The immunoprecipitated proteins showed successful enrichment of target protein, with reduced nonspecific background and less contamination from eluted antibodies. To further improve protein recovery, the hydrophobic poly(PFPA) brush was modified with hydrophilic poly(ethylene glycol) (PEG). The partially PEG-substituted poly(PFPA) brush showed better dispersion in aqueous solution, leading to improved antibody immobilization efficiency. By optimizing both the brush molecular weight and the degree of PEG substitution, an optimal balance between surface hydrophilicity and number of available PFP units was found, leading to efficient target protein purification. This study shows that poly(PFPA) platform offers a versatile approach to prepare biomolecule-activated surfaces with tunable surface property, which has potential applications in protein separation and other areas.
Publisher
AMER CHEMICAL SOC
Issue Date
2018-03
Language
English
Article Type
Article
Keywords

A-ANTIBODY ADSORBENT; POLYMER BRUSHES; REVERSIBLE ADDITION; RADICAL POLYMERIZATION; GRAFTING DENSITY; CROSS-LINKING; THIN-FILMS; IN-VIVO; IMMOBILIZATION; SURFACE

Citation

BIOMACROMOLECULES, v.19, no.3, pp.951 - 961

ISSN
1525-7797
DOI
10.1021/acs.biomac.7b01736
URI
http://hdl.handle.net/10203/241218
Appears in Collection
CBE-Journal Papers(저널논문)
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