Mechanism-based probe for the analysis of cathepsin cysteine proteases in living cells

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Mechanism-based probes are providing new tools to evaluate the enzymatic activities of protein families in complex mixtures and to assign protein function. The application of these chemical probes for the visualization of protein labeling in cells and proteomic analysis is still challenging. As a consequence, imaging and proteomic analysis often require different sets of chemical probes. Here we describe a mechanism-based probe, azido-E-64, that can be used for both imaging and proteomics. Azido-E-64 covalently modifies active Cathepsin (Cat) B in living cells, an abundant cysteine protease involved in microbial infections, apoptosis, and cancer. Furthermore, azido-E-64 contains an azide chemical handle that can be selectively derivatized with phosphine reagents via the Staudinger ligation, which enables the imaging and proteomic analysis of Cat B. We have utilized azido-E-64 to visualize active Cat B during infection of primary macrophages with Salmonella typhimurium, an facultative intracellular bacterial pathogen. These studies demonstrated that active Cat B is specifically excluded from Salmonella-containing vacuoles, which suggests that inhibition of protease activity within bacteria-containing vacuoles may contribute to bacterial virulence.
Publisher
AMER CHEMICAL SOC
Issue Date
2006
Language
English
Article Type
Article
Keywords

COPPER(I)-CATALYZED AZIDE-ALKYNE; SALMONELLA-CONTAINING VACUOLES; DEUBIQUITINATING ENZYME; CONTAINING PHAGOSOMES; III SECRETION; TYPHIMURIUM; MACROPHAGES; LYSOSOMES; STRATEGY; PROTEIN

Citation

ACS CHEMICAL BIOLOGY, v.1, no.11, pp.713 - 723

ISSN
1554-8929
DOI
10.1021/cb600431a
URI
http://hdl.handle.net/10203/240861
Appears in Collection
MSE-Journal Papers(저널논문)
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