In this study, the intervention of bacterial communication or quorum quenching (QQ) technique has been investigated to mitigate biofouling in forward osmosis (FO) membrane processes. The 10 mg-C/L lysate of QQ enzyme-producing Rhodococcus sp. BH4 successfully degraded 79% of the bacterial signal molecule (N-acyl homoserine lactone, AHL). In a continuous lab-scale FO experiment using Pseudomonas aeruginosa PAO1 as a model bio-foulant, flux recovery after physical cleaning was higher in the presence of 10 mg/L of Rhodococcus lysate. The retardation of biofouling in the presence of Rhodococcus lysate was largely due to the reduced amount of bio-volume and extracellular polymeric substances (EPS), which were reduced by 68% and 75% compared with the control. In conclusion, the application of bacteria-oriented QQ molecules could be the potential solution not only to mitigate biofouling but also to meet the economic demands.