Fed-batch Culture of Aeromonas hydrophila for the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) using two carbon sources

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To produce polyhydroxyalkanoate (PHA) copolymer which consists of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx) by cultivation of Aeromonas hydrophila, fed-batch cultures were done under several nutrient limiting conditions. With the results from flask cultures, fed-batch cultures were carried out to produce large amounts of PHA. In the fed-batch culture, firstly glucose was fed to grow cell, and then, oleic acid fed to stimulate PHA in the cell. The final cell concentration, PHA content, PHA concentration, and 3-hydroxy-hexanoate fraction in 38 hr were 48.9 g/L, 15.05 wt%, 7.36 g/L and 12.2 wt%, respectively, resulting in the productivity of 0.19 g/L-h under phosphate-limiting condition.
Publisher
Korean Society for Biotechnology and Bioengineering
Issue Date
1999
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.4, no.3, pp.195 - 198

ISSN
1226-8372
URI
http://hdl.handle.net/10203/24070
Appears in Collection
CBE-Journal Papers(저널논문)
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